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photo of biological safety cabinet

A Biolgical Safety Cabinet (BSC) is an enclosed, ventilated lab workspace for safely handling materials that are contaminated (or potentially contaminated) with pathogens. BSCs are often used to maintain aseptic conditions.

 

 

 

Class

I

II

III

Type

 

A1

A2 (A/B3)

B1

B2

 

Face Velocity (lfm)

75

75

100

100

100

N/A

Recirculated Air

0%

70%

70%

30%

0%

0%

Exhaust Air

100%

30%

30%

70%

100%

100%

Ducting

Hard

Unducted

Thimble

Unducted

Thimble

Hard

Hard

Hard

Use Volatile Chemicals

Small Amounts

No

Minute Amounts

No

Minute Amounts

Minute Amounts

Small Amounts

Yes

Personnel/
Environmental Protection

Yes

Yes

Yes

Yes

Yes

Yes

Product Protection

No

Yes

Yes

Yes

Yes

Maybe

Continuous Inward Airflow protects the user from aerosols.

Filtered Downward Laminar Airflow & Exhaust
BSCs use High-Efficiency Particulate Air (HEPA) filters.

  • The minimum efficiency is 99.97% at 0.3 µm.
  • HEPA Filters do not remove gasses or vapors

Ducting and Alarms

Hard-ducted BSCs exhaust through the building’s fume hood exhaust system.

Thimble-ducted (canopy) exhaust draws air from both the BSC and the room.

Unducted BSCs exhaust HEPA-filtered air back into the room.

Alarms are required on ducted BSCs to notify the user if the building’s exhaust system fails. In a hard-ducted BSC, immediately stop work and close the sash when the alarm sounds.

BSCs must be certified per NSF/ANSI 49 Standards

  • When new (before first use)
  • After repair/service
  • Every year
  • If relocated (even within the same room)

Contact OEHS at 1-801-581-6590.

BSCs should be located away from other sources of airflow such as doors, windows, supply air vents, high-traffic areas and other lab equipment (e.g., BSCs, fume hoods, incubators). Consult with OEHS Biosafety on room layout plans before installing BSCs to ensure that the proposed location does not compromise protection

  • Accessories: Open flames are not permitted in BSCs because they disrupt airflow, damage the HEPA filter, and can lead to a build-up of flammable gases. Ordering BSCs without gas ports is recommended.
  • Lighting: The use of UV light in BSCs is strongly discouraged by the NIH, CDC and the University of Utah. If UV light is used, personnel must leave the room while the light is on.
  • Customization: BSCs can be customized at the factory to accommodate, e.g., microscope face pieces, large equipment, dunk tanks or cage  changing. Consult with vendors about your needs to identify options.
  • Materials: Bleach and other corrosive chemicals can damage steel surfaces. Follow bleach decontamination by wiping the surface with 70% ethanol or sterile water to protect against corrosion.
  • Sizing: BSCs should have several inches of clearance from the wall(s) and other equipment and are designed for use by one person at a time.  Consider multiple smaller BSCs instead of one larger BSC if simultaneous work scheduling is anticipated.

 

Adapted from UCLA EH&S guidance

 

 

 

 

Last Updated: 3/28/24